IIn vitro study of green tea, tangerine pulp and prickly pear cactus extracts on platelet aggregation, RBC Na‐Leak and TBARS production

Abstract

We compared the radical scavenging activity of the extracts of green tea, prickly pear cactus, tangerine pulp and vitamin E and their invitro effects of antioxidant on erythrocyte Na leak, platelet aggregation and TBARS production using SD rats. Platelet aggregation incubated with green tea extract was significantly decreased in the maximum and initial slope compared with other extracts or vitamin E (p<0.05). Na leak in AAPH treated erythrocyte was significantly lower when incubated with cactus extract compared with other extracts or vitamin E. Hemolysis of AAPH treated RBC was significantly lower when incubated with vitamin E compared with control and the extracts (p<0.05). TBARS production in AAPH treated platelet rich plasma (PRP) was significantly increased compared to the untreated PRP (p< 0.01), and vitamin E significantly decreased TBARS production in the AAPH treated PRP (p<0.01). Vtamin E significantly decreased TBARS production in the AAPH treated human LDL compared with all other groups (p<0.01). Present study showed that green tea in vitro system has lowering effect of platelet aggregation and vitamin E has a protective effect on membrane preventing lipid peroxidation and membrane damage.