Abstract
1) The effects of inhibitors on the endogenous respiration of various tissues and the identification of cytochromes in various tissues of fresh-water cockle, Corbicula sandai, were studied. 2) The respiration of gill, mantle and adductor muscle tissues was inhibited by 5×10-4 M cyanide or 10-3 M azide and the inhibition was reversed by the addition of 5×10-3 M methylene blue (Table 1). 3) In a gas mixture of 95% CO and 5% O2, the oxygen uptake of gill and mantle tissues was inhibited by about 50% in the dark and the inhibition was completely reversed by light (Fig. 1 and Table 2). 4) Cytochrome oxidase activity of gill and mantle tissues was measured manometrically (Table 3). The absorption band of reduced cytochrome b was detected in all tissues examined, but the bands corresponding to cytochromes a and c were not detected at room temperature. A feeble bands of cytochromes a and c appeared at liquid-air temperature (Table 4). 5) These results lead to the conclusion that the respiration of fresh-water cockle tissues is mediated through the cytochrome system.
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