Abstract
A method is described which permits the simultaneous isolation and separation of insoluble aminoacyl-tRNA synthetase protein · RNA complexes containing high specific synthetase activity, and soluble tRNA ∗ ∗ tRNA means amino acid accepting RNA. Soluble RNA or soluble tRNA means RNA found in fractions from chromatography on DEAE-cellulose containing no insoluble material. which retains 25% to 50% of its specific amino acid accepting activity. A possible amino acid accepting activity of the RNA part of the insoluble aminoacyl-tRNA synthetase protein · RNA complex was investigated by assaying the unchanged complex and the RNA obtained after dissociation from the protein part of the synthetase complex. No amino acid accepting activity was found.
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