Abstract
Recently, an assay method for the determination of norepinephrine and dopamine in biological specimens by application of high performance liquid chromatography with electrochemical detection has been developed. Application of a microparticle reversed-phase column has made clean separation and detection of trace amounts of each amine possible. This paper presents an assay method for the simultaneous measurement of norepinephrine, dopamine and epinephrine with N-methyldopamine as the internal standard. Incorporation of an effective sensitivity-shift technique resulted in a remarkable increase in sensitivity with this method. The assay limit for quantitation was approximately 50 pg for all amines. Applicability was also studied following administration of reserpine or fusaric acid.
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