III. Reversed phase high pressure liquid chromatography for the identification and purification of neuropeptides

Abstract

Reversed phase HPLC has wide applications in studies on neuropeptides. It provides a fast and effective technique for assessing the purity of synthetic peptides and for purifying mg amounts of synthetic peptides (examples: angiotensins II and III and analogues; neurohypophysial hormones). Due to the very small quantities of peptides which can usually be safely recovered after HPLC, the method is also useful in the isolation, purification and sequencing of peptides from biological sources (examples: urotensins I and II), and in the identification of neuropeptides in tissues when coupled with radioligand-binding displacement assays (example: [arginine 8]vasotocin in the anterior ganglia of Aplysia california ).