Abstract
Methods for very efficient isolation of non-degraded chloroplasts from Antirrhinum majus are described. When studied in the analytical ultracentrifuge, isolated ribosomes of such chloroplasts show a single symmetrical 68s peak. Extraction of the ribosomal RNA from chloroplasts and chromatographic separation on methylated serum albumin yields 4 main fractions: (1) low molecular weight RNA (fraction I b) with s20 = 5.5, (2) high molecular weight RNA (fraction III) with s20 = 16-17, (3) high molecular weight RNA (fraction V) with s20 = 23, and (4) a RNA fraction (fraction VI) heterogeneous in molecular size. In addition data are presented which show a higher resolution of RNA species by polyacrylamide gel electrophoresis than by chromatographic fractionation on methylated serum albumin. The high molecular weight RNA (16 -17s) of chloroplasts differs from the corresponding “light” ribosomal RNA of the cytoplasm in its electrophoretic mobility.
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