IGFBP2 is a potential biomarker in acute kidney injury (AKI) and resveratrol-loaded nanoparticles prevent AKI.

Hai-Lun Li,Yong-Tao Lin,Dong-Hui Zheng,Xiao-Feng Tian,Zhuan Yan,Zun-Ping Ke,Li-Li Zhong,Yong Xu

doi:10.18632/oncotarget.25663

Abstract

This study aims to determine whether insulin-like growth factor binding protein2 (IGFBP2) is a useful biomarker for early diagnosis of acute kidney injury (AKI), evaluate the therapeutic effects of resveratrol-loaded nanoparticles (Res-NPs), and investigate the possible underlying mechanisms in a rat model of AKI induced by IRI. Forty male Sprague–Dawley rats were randomly divided into four groups (10 animals per group): sham, IRI control, resveratrol, and Res-NPs injection. Kidney injury and the effects of Resveratrol and Res-NPs were determined by histological examination, renal function, cell apoptosis profile, and gene expression. Changes in IGFBP2 were similar with the pattern of well-known renal biomarkers, namely, kidney injury molecule 1 and neutrophil gelatinase-associated lipocalin, in all groups. Compared with the IRI control and resveratrol groups, the Res-NPs groups displayed significantly reduced apoptotic rate, reactive oxygen species level, and malondialdehyde content, downregulated protein expression levels of Caspase3 and Bax with increased antioxidant glutathione peroxidase level, and upregulated expression of Bcl-2 protein. Thus, IGFBP2 may serve as a promising novel biomarker of AKI, and Res-NPs may prevent kidney injury from ischemia/reperfusion in a rat model.

Highlights

Acute kidney injury (AKI), is defined as acute reduction in glomerular filtration rate occurring over hours to days, with a subsequent rise in serum creatinine concentrations
Our study clearly demonstrated for the first time that insulin-like growth factor binding protein2 (IGFBP2) like kidney injury molecule 1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL) may be a reliable diagnostic and prognostic biomarker for acute kidney injury (AKI)
This finding is consistent with our results in which KIM-1 and NGAL expression levels were increased triggered by ischemia and declined with the recovery of renal injury with the treatment of Resveratrol and ResNPs injection

Summary

Introduction

Acute kidney injury (AKI), is defined as acute reduction in glomerular filtration rate occurring over hours to days, with a subsequent rise in serum creatinine concentrations. AKI is a common and serious complication among a large range of human diseases characterized with a rapid decline in kidney function. The incidence of AKI in hospitalized population employing a Kidney Disease: Improving Global Outcome-equivalent criteria for AKI diagnosis was from 16.9% to 31.0% in Western countries [1] and from 7.5% to 31.0% in Asian areas [2]. With its increase in the older population, the prevalence of AKI will continue to rise. Early diagnosis of AKI is often difficult and its therapy is lacking

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