Abstract
As a disease characterized by severe liver necrosis and hemorrhage, duck viral hepatitis (DVH) is mainly caused by duck hepatitis A virus (DHAV). The positive-strand RNA genome of DHAV type 1 (DHAV-1) contains an internal ribosome entry site (IRES) element within the 5′ untranslated region (UTR), structured sequence elements within the 3′ UTR, and a poly(A) tail at the 3′ terminus. In this study, we first examined that insulin-like growth factor-2 mRNA-binding protein-1 (IGF2BP1) specifically interacted with the DHAV-1 3′ UTR by RNA pull-down assay. The interaction between IGF2BP1 and DHAV-1 3′ UTR strongly enhanced IRES-mediated translation efficiency but failed to regulate DHAV-1 replication in a duck embryo epithelial (DEE) cell line. The viral propagation of DHAV-1 strongly enhanced IGF2BP1 expression level, and viral protein accumulation was identified as the key point to this increment. Collectively, our data demonstrated the positive role of IGF2BP1 in DHAV-1 viral proteins translation and provided data support for the replication mechanism of DHAV-1.
Highlights
Duck viral hepatitis (DVH), firstly described in Long Island in 1949 [1], is an acute and highly fatal disease of young ducklings characterized by severe liver necrosis, hemorrhage, and high mortality
insulin-like growth factor-2 messenger RNA (mRNA)-binding protein-1 (IGF2BP1) Interacted with 30 untranslated region (UTR) of duck hepatitis A virus (DHAV)-1
IGF2BP1 interacted with the Duck hepatitis A virus type 1 (DHAV-1) 30 UTR (Figure 2)
Summary
Duck viral hepatitis (DVH), firstly described in Long Island in 1949 [1], is an acute and highly fatal disease of young ducklings characterized by severe liver necrosis, hemorrhage, and high mortality. This disease is caused by duck hepatitis virus (DHV) types 1, 2, and 3, and there were no antigenic relationships among them [2,3]. Duck hepatitis virus types 1 (DHV-1) was classified as a member of Picornaviridae and renamed as duck hepatitis A virus (DHAV) according to the Virus. Duck hepatitis A virus type 1 (DHAV-1) possesses a positive, single-stranded, non-enveloped RNA genome of ~7700 nucleotides (nt), including the 50 untranslated region (UTR), one open reading frame (ORF), and 30 UTR following with poly(A) tail [5].
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