Abstract
The age-associated decline in skeletal muscle’s ability to regenerate is a concern among elderly patients of limb injury or orthopedic surgery. Changes in insulin-like growth factor-I (IGF-I) response have been implicated in this phenomenon. Previously, our laboratory has shown a drastic increase in IGF-I mRNA 7 days following tourniquet (TK) induced damage in adult rats, a response not seen in elderly rats. This study investigated the short-term time course of IGF-I expression in adult (6 month-old) and elderly (24 month-old) mice following 1, 3, 5, and 7-day recovery from 2 hour TK induced ischemia/reperfusion injury. RT-PCR analysis reveals an increase in IGF-I expression in both TK groups. Levels peaked ~20-fold 3 days post-injury in adult and ~8-fold 5 days post-injury in elderly subjects. Adult expression decreased sharply at day 5 and rose by day 7, suggesting the differential, temporal-specific expression of the IGF-I Ea and Eb isoforms in injury repair described by Goldspink et al. The early IGF-I response seen in adult subjects was not present in elderly subjects.
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