IgE Recognition Pattern of Homologous Allergens Tested by Microarray-based Nanotechnology

Abstract

RATIONALE: IgE recognition of homologous molecules shared by inhalant and food allergen sources are often seen in clinical practice. The aim of our study was to evaluate through allergen microarray technique the IgE recognition pattern of the allergic population.METHODS: 1110 individuals (42/58 male/female) with a clinical history of urticaria, rhinitis, asthma and atopic dermatitis have been recruited and tested by allergen-based microarray system (VBC-Genomic, Vienna, Austria). Patients were grouped on the basis of their reactivity to at least one allergen in one of the following three groups: Profilins (Bet v 2, Cyn d 12, Hel a 2, Hev b 8, Mer a 1, Ole e 2, Par j 3, Phl p 12, Pho d 2); Bet v 1-related allergens (Aln g 1, Api g 1, Bet v 1a, Bet v 1d, Cor a 1, Dau c 1 and Mal d 1.0108); tropomyosins (Ani s 3, Der p 10, Hel as 1, Pen i 1, Pen m 1, Per a 7).RESULTS: 523 (47%) subjects were positive to at least one Bet v 1-related allergens, 415 (37%) to at least one Profilin, 123 (11.1%) to at least one tropomyosin. Bet v 1 (85%) was the most frequently IgE positive molecule within Bet v 1-related allergen reactive individuals, whereas Mer a 1 (89%) ranked first within the profilin subset, and Der p 10 (80%) was the most IgE recognized tropomyosin. Stratifying subjects by IgE concentrations showed an almost homogeneous recognition pattern for all profilins and tropomyosins, whereas IgE to some Bet v 1-like molecules from foods were not detected even at high Bet v 1-IgE concentration.CONCLUSIONS: Panel of grouped allergens to be tested by means of microarray system are helpful to fully describe homologous allergenic molecule immunological relationship. IgE reactivity pattern could relate to patient clinical picture. RATIONALE: IgE recognition of homologous molecules shared by inhalant and food allergen sources are often seen in clinical practice. The aim of our study was to evaluate through allergen microarray technique the IgE recognition pattern of the allergic population. METHODS: 1110 individuals (42/58 male/female) with a clinical history of urticaria, rhinitis, asthma and atopic dermatitis have been recruited and tested by allergen-based microarray system (VBC-Genomic, Vienna, Austria). Patients were grouped on the basis of their reactivity to at least one allergen in one of the following three groups: Profilins (Bet v 2, Cyn d 12, Hel a 2, Hev b 8, Mer a 1, Ole e 2, Par j 3, Phl p 12, Pho d 2); Bet v 1-related allergens (Aln g 1, Api g 1, Bet v 1a, Bet v 1d, Cor a 1, Dau c 1 and Mal d 1.0108); tropomyosins (Ani s 3, Der p 10, Hel as 1, Pen i 1, Pen m 1, Per a 7). RESULTS: 523 (47%) subjects were positive to at least one Bet v 1-related allergens, 415 (37%) to at least one Profilin, 123 (11.1%) to at least one tropomyosin. Bet v 1 (85%) was the most frequently IgE positive molecule within Bet v 1-related allergen reactive individuals, whereas Mer a 1 (89%) ranked first within the profilin subset, and Der p 10 (80%) was the most IgE recognized tropomyosin. Stratifying subjects by IgE concentrations showed an almost homogeneous recognition pattern for all profilins and tropomyosins, whereas IgE to some Bet v 1-like molecules from foods were not detected even at high Bet v 1-IgE concentration. CONCLUSIONS: Panel of grouped allergens to be tested by means of microarray system are helpful to fully describe homologous allergenic molecule immunological relationship. IgE reactivity pattern could relate to patient clinical picture.