Abstract
BackgroundMany studies have investigated the characteristics and biological activities of type III interferon (IFN), finding that it has similar features to type I IFN but also unique actions because it is recognized by a different receptor.ResultsA full-length recombinant human IFN-λ1 (rhIFN-λ1) cDNA was cloned into the pDF expression vector and stably expressed in Flp-In-CHO cells. After four purification steps (ammonium sulfate precipitation, SP Sepharose chromatography, Blue Sepharose 6 fast flow affinity chromatography and molecular sieve chromatography), the rhIFN-λ1 had a purity of about 90% and was found to have the predicted biological activities. The anti-viral activity of rhIFN-λ1 was determined as 106 IU/mg using the vesicular stomatitis virus (WISH-VSV) assay system. The anti-proliferation activity of rhIFN-λ1 was measured using the MTS method and the growth inhibition ratio was 57% higher than that for recombinant human IFN-α2b (rhIFN-α2b) when the rhIFN-λ1 concentration was 1000 IU/ml. rhIFN-λ1 had lower natural killer cell cytotoxicity than rhIFN-α2b.ConclusionThe Flp-In-CHO system is suitable for stably expressing rhIFN-λ1 that possesses the predicted anti-viral, anti-proliferation and natural killer cell cytotoxicity-promoting activities.
Highlights
In 2003, a new type of interferon (IFN) group, which contains the molecules IFN-λ1, IFN-λ2 and IFN-λ3, was identified by two different research groups [1, 2]
Type III and type I IFNs have similar biological activities, including anti-viral, anti-proliferative and antitumor activities, but they have different receptors: an IFN-αR1:IFN-αR2 heterodimer for type I IFNs and an IFN-λR1:IL10R2 heterodimer for type III IFNs [3]
In contrast to the IFN I receptor subunits and the IL-10R2 chain, which are ubiquitously expressed, the IFN-λR1 chain is primarily expressed by epithelial cells and dendritic cells, restricting the action of type III IFNs to these specific cell types [4, 5]
Summary
In 2003, a new type of interferon (IFN) group, which contains the molecules IFN-λ1, IFN-λ2 and IFN-λ3, was identified by two different research groups [1, 2]. These IFNs belong to the type III IFN family. Many type I IFN-based molecules have been widely used in clinical therapy. Many studies have investigated the characteristics and biological activities of type III interferon (IFN), finding that it has similar features to type I IFN and unique actions because it is recognized by a different receptor
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