Abstract
IFN-γ is a cytokine which functions in a wide range of biological activities by inducing a number of early and delayed genes. In murine IL-3-dependent cell lines BAF-B03 and 32D, IFN-γ upregulatedbag-1 andbcl-xLgene expression. These cells revealed prolonged cell survival against IL-3-deprivation by IFN-γ stimulation. In contrast, human myeloma cell line RPMI8226, despite expression of IFN-γ receptor, showed neither induction of their expressions nor prolonged cell survival against serum starvation-induced apoptosis by IFN-γ stimulation. Gene-transfer-mediated overexpression of BAG-1 protein in BAF-B03 cells led to prolonged cell survival against IL-3-deprived apoptosis compared with control BAF-B03 transfectants, indicating that levels of BAG-1 expression are crucial for cell survival in BAF-B03 cells. Taken together, these studies suggest that induction of anti-apoptotic gene expression is a crucial factor for the anti-apoptotic function of IFN-γ in IL-3-dependent immature hematopoietic cells.
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