IFN‐γ Inhibits Osteopontin Expression in Human Decidual Stromal Cells and can be Attenuated by 1α,25‐Dihydroxyvitamin D3

Abstract

The effect of IFN-γ on the expression of osteopontin (OPN), in the presence or absence of 1α,25-dihydroxyvitamin D3 (1,25(OH)(2) D(3) ), in decidual stromal cells (DSC). Decidual stromal cells were isolated from women undergoing elective termination of pregnancy (gestational age, 6-9weeks). After characterization, they were treated with IFN-γ in the presence or absence of 1,25(OH)(2) D(3) . The uterus of pregnancy IFN-γ knockout mice were collected on gestation day (gd) 7.5, and the expression of OPN were examined. IFN-γ drastically decreased the expression of OPN in DSC, which was reverted by the addition of 1,25(OH)(2) D(3) to the IFN-γ-treated decidual cells. Moreover, the OPN expression in uterus of IFN-γ knockout mice was higher than that of wild-type counterparts. We demonstrated OPN was expressed in DSC in human first-trimester decidua and in the uterus in mice at 7.5gd. The OPN expression was closely correlated with regulation of IFN-γ and 1,25(OH)(2) D(3) in human early pregnancy. OPN expression in DSC was significantly decreased with the treatment of IFN-γ. 1,25(OH)(2) D(3) played an opposite role in IFN-γ-mediated inhibition of OPN expression in human DSC.