Idiopathic Recurrent Pregnancy Loss related toGPIagenein Iraqi patient women

Abstract

Background: Recurrent pregnancy loss (RPL) is a major concern in the field of reproductive medicine. In particular, idiopathic recurrent pregnancy loss (iRPL) is a condition in which recurrent pregnancy loss occurs without a known cause. Recent studies have shown that genetic factors may contribute to iRPL, and the presence of the 807C/T silent polymorphisms in the GPIa gene has been associated with a higher risk of thrombosis and recurrent abortion.Aim: to determine the allelic frequency of GPIa 807C/T in patients with iRPL and healthy controls in Iraq.Material and Method: A T-ARMS PCR-based method was used to analyze the allelic frequency of GPIa 807C/T in 25 patients with iRPL and 25 healthy controls. The genotypic and allelic frequencies were calculated, and a statistical analysis was performed.Result: The results of this study showed that the allelic frequency for 807C was 48% and 46% in the patient and control groups, respectively. The allelic frequency of 807T was recorded as 52% in the patient group, whereas the control group showed 54%. The patient group's genotypic frequencies were distributed as 16% for 807TT, 12% for 807CC, and 72% for 807CT. On the other hand, the control group reported genotypic frequencies of 32% for 807TT, 24% for 807CC, and 44% for 807CT. Interestingly, the TC heterozygous genotype of the GPIa gene's 807C\T showed a significant increase (P ≤ 0.05) with a risk (OR=3.27) in the patient group against the control.Conclusion: the results of this study suggest that the presence of the GPIa 807C/T polymorphism is associated with a higher risk of iRPL in Iraqi patient women. The findings of this study could be used to develop new diagnostic and therapeutic strategies for iRPL.