IDF21-0098 Curcumin prevents renal aggravation by ischemia and reperfusion in diabetes mellitus

Abstract

Background: Diabetes Mellitus (DM) increases the susceptibility to ischemia and reperfusion (I/R). I/R and DM share common physiopathological mechanisms, like inflammation and oxidation. Curcumin, present in Curcuma longa l., is a natural anti-inflammatory and antioxidant, used as a medicine for the treatment of osteoarticular diseases and usually found as a food, in view of its regulation of practical use, it is considered to be used in the prevention of acute chronic kidney disease. Aim: The aim of this study was to evaluate the effect of previous Curcumin treatment on renal function, hemodynamics and oxidative profile of diabetic rats submitted to I/R. Method: Wistar male rats (250–290 g), randomized into four groups: Citrate- received citrate buffer, i.v., on 1st day; DM: received 65 mg/kg streptozotocin (STZ), i.v., diluted in citrate buffer, on 1st day; DM+I/ R: diabetic animals that on 26th day were submitted bilateral renal pedicle clamping for 30 min; DM+I/R+ Curcumin: diabetic animals that received Curcumin 30mg/kg/day, gavage, 17th until the 27th day, and on the 26th day were submitted I/R. The renal function (inulin clearance- InCl, urinary flow- UF, serum creatinine- SCr), renal hemodynamics (renal blood flow -RBF, renal vascular resistance- RVR) and oxidative profile (urinary peroxides- UP, urinary nitrate- UN, thiobarbituric acid reactive substances- TBARS and thiols in renal tissue) were assessed. Results: Curcumin treatment in diabetic animals submitted to I/R insult resulted in increased InCl and decreased SCr, accompanied by increased RBF and decreased RVR with a reduction in UP, UN, TBARS and increase in thiol renal. Discussion: Curcumin prevented renal function decline in diabetic rats submitted to I/R with beneficial repercussions on renal hemodynamics and renal oxidative profile compared with control rats.