Abstract
Seventy-one Neurospora cultures were isolated from Oncom tahu based on soybean residues in the Jakarta-Bogor and from Oncom based on peanut presscake in the Lembang-Bandung regions of West Java. They were heterothallic and had 8-ascospore per ascus. In crosses with authentic reference cultures of three known heterothallic species, Neurospora intermedia, N. crassa and N. sitophila, only crosses with N. intemedia produced abundant viable black ascospores and few inviable white ones. This meiotic sterility test of hybrids identified the Oncom cultures as N. intermedia Tai and not N. sitophilia as previously assumed. However, all the Oncom isolates except one (H2118) differed from wild N. intermedia collected from remains of burnt vegetation. The Oncom cultures with the proposed varietal status as N. intermedia var. oncomensis had bright yellow and large macroconidia in contrast to wild N. intermedia and H2118 with pink and small macroconidia. Some of the Oncom cultures showed degeneration towards poor mycelial growth and loss of conidiation on repeated vegetative subculturing.
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