Identifying the RNA Binding Sites on the MeCP2 Protein by Regional Deletion

Abstract

Long non-coding RNAs (LncRNAs) have been found to bind to the Methyl-CpG binding protein (MeCP2), which has been speculated to play a role in altering chromatin structure and regulating gene expression.  Whether MeCP2 has other important functions related to RNA processing, the nature of the LncRNA-MeCP2 interaction and function, and how this may affect brain development is unknown.  The goal of my project is to provide insight into important regions or domains of the MeCP2 protein involved in RNA binding.  With this new information, a foundation for further in-depth research will be established, on the involvement of the MeCP2 protein in the pathogenesis of Rett Syndrome, through RNA binding and RNA regulation. For the full text, please visit https://scholar.colorado.edu/concern/undergraduate_honors_theses/mw22v689v.