Abstract

Mms2 is an ubiquitin E2 variant that works with the E2 conjugating enzyme Ubc13 in the DNA damage response pathway. To gain a better understanding of the functions of Mms2 as well as the molecular mechanisms that regulate Mms2, we conducted a proteomic study to identify proteins that interact with Mms2. Flag‐tagged Mms2 was expressed in the budding yeast, and proteins that co‐immunoprecipitated with Flag‐tagged Mms2 were analyzed by mass spectrometry. As a result, we identified Mpk1/Slt2, a mitogen activated protein kinase (MAP kinase), as an interacting protein of Mms2. Notably, Mms2 protein contains a serine residue that matches the consensus sequence (PXSP) of MAP kinase, suggesting a possibility that Mms2 is a substrate of Mpk1/Slt2. Studies designed to test this hypothesis are underway.

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