Abstract
Soybean mosaic virus (SMV) is detrimental to soybean (Glycine max) breeding, seed quality, and yield worldwide. Improving the basic resistance of host plants is the most effective and economical method to reduce damage from SMV. Therefore, it is necessary to identify and clone novel SMV resistance genes. Here, we report the characterization of two soybean cultivars, DN50 and XQD, with different levels of resistance to SMV. Compared with XQD, DN50 exhibits enhanced resistance to the SMV strain SC7. By combining bulked-segregant analysis (BSA)-seq and fine-mapping, we identified a novel resistance locus, RSMV-11, spanning an approximately 207-kb region on chromosome 11 and containing 25 annotated genes in the reference Williams 82 genome. Of these genes, we identified eleven with non-synonymous single-nucleotide polymorphisms (SNPs) or insertion-deletion mutations (InDels) in their coding regions between two parents. One gene, GmMATE68 (Glyma.11G028900), harbored a frameshift mutation. GmMATE68 encodes a multidrug and toxic compound extrusion (MATE) transporter that is expressed in all soybean tissues and is induced by SC7. Given that MATE transporter families have been reported to be linked with plant disease resistance, we suggest that GmMATE68 is responsible for SC7 resistance in DN50. Our results reveal a novel SMV-resistance locus, improving understanding of the genetics of soybean disease resistance and providing a potential new tool for marker-assisted selection breeding in soybean.
Highlights
Soybean [Glycine max (L.) Merr.] is one of the most important sources of plant protein and vegetable oil, providing more than one-quarter of the world’s protein for food and animal feed (Hoeck et al, 2003; Boerma and Specht, 2004)
To assess the variation in disease resistance between DN50 and XQD, we identified the phenotype of DN50 and XQD
The accumulation of viral CP gene in DN50 was significantly lower than that in XQD (Figure 1F). These results suggested that DN50 is more resistant than XQD to SC7
Summary
Soybean [Glycine max (L.) Merr.] is one of the most important sources of plant protein and vegetable oil, providing more than one-quarter of the world’s protein for food and animal feed (Hoeck et al, 2003; Boerma and Specht, 2004). Breeding soybean varieties with both high seed quality and high yield remains an important goal of breeders. Soybean mosaic virus (SMV), a single-stranded, positive-sense RNA virus of the genus Potyvirus, causes losses in soybean yields and seed quality worldwide (Ross, 1977; Hajimorad et al, 2018). A series of Rsv loci have been reported in soybean accessions and introduced into commercial varieties: Resistance to SMV1 (Rsv1), Rsv, Rsv, and Rsv (Kiihl and Hartwig, 1979; Buss et al, 1997; Hayes et al, 2000; Klepadlo et al, 2017). Rsv confers resistance to SMV G5–G7strains (Gunduz et al, 2002). Rsv confers broad-spectrum SMV resistance through an atypical mechanism that delays viral proliferation (Gunduz et al, 2004). The Rsv locus was first reported in 1995, and its molecular characterization has recently been achieved: it encodes an RNase H family protein with double-stranded (ds) RNA-degrading activity that enters the viral replication compartment and degrades viral dsRNA (Ishibashi et al, 2019)
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