Identifying novel mediators of glioblastoma invasion in a hyaluronic acid-based microscale platform with CRISPR screening

Abstract

Glioblastoma (GBM) is the most common and aggressive form of primary brain cancer, with a devastatingly low median survival time of 15 months despite surgical resection and chemoradiotherapy. GBM is characterized by rapid and highly invasive growth in which individual or collective cancer cells migrate away from the central tumor into the surrounding healthy brain tissue. The diffuse invasion pattern of GBM makes complete surgical resection nearly impossible and inevitably leads to tumor recurrence.