Abstract
Most studies on embryonic metabolism had been solely focused on glucose utilization and amino acid turnover. Thus, un-intended metabolic pathways were not able to be identified. An untargeted metabolomic profiling aims at holistically identifying all available metabolome in a one shot study. We used UHPLC-ToF-MS with chemomatrics to analyze spent embryo culture media by drops. Critical metabolites and relevant metabolic pathways differentiating viable embryos from non-viable embryos were investigated noninvasively.
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