Identifying Biomarkers Associated with Pain in Swine using an In Vitro Inflammation Model

Abstract

Event Abstract Back to Event Identifying Biomarkers Associated with Pain in Swine using an In Vitro Inflammation Model Michael J. Myers1*, Christine M. Deaver1, Elzabeth A. Kenyon1, Sharla M. Peters1, Heidi L. Swaim1, Katyln Tanke1 and Haile F. Yancy1 1 US Food and Drug Administration/Center for Veterinary Medicine, Division of Applied Veterinary Research, United States This study sought to determine if biomarkers associated with pain could be measured in an in vitro model of inflammation. Success would constitute the first step towards using these biomarkers as surrogate endpoints to help support effectiveness indications for investigational new animal drugs to control pain in swine. Porcine whole blood samples were cultured in vitro with E. coli lipopolysaccharide (LPS) or without LPS for up to 48 hr. Supernatant from these cultures was collected to determine protein levels. Messenger RNA was also obtained from these cells and quantitative reverse transcription-PCR was used to determine if mRNA levels of genes associated with pain were altered in response to LPS-induced inflammation. Bradykinin protein levels steadily increased over time due to LPS stimulation and returned to 0 hr levels after 6 hr of culture. Corticotrophin-releasing factor protein levels were not affect by LPS. Substance-P protein levels steadily increased over time and returned to 0 hr levels after 4 hr LPS stimulation. Genomically, Chemokine Receptor 4 and Bradykinin Receptor 1 had a 3-fold fold increase at 6 hr after LPS stimulation. Cyclin-Dependent Kinase Inhibitor 1A showed a 4 fold increase at 6 hr and 24 hr after LPS stimulation. An additional 17 genes associated with pain showed no alteration in mRNA levels in this model. These results suggest that 5 biomarkers (2 protein and 3 genomic) may be useful as surrogate endpoints for evaluation of pain. These need to be evaluated in vivo to support their usefulness as biomarkers of pain in swine. Keywords: Inflammation, biomarkers, Pain, Swine, Innate immune system Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Myers MJ, Deaver CM, Kenyon EA, Peters SM, Swaim HL, Tanke K and Yancy HF (2013). Identifying Biomarkers Associated with Pain in Swine using an In Vitro Inflammation Model. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00497 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Apr 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Michael J Myers, US Food and Drug Administration/Center for Veterinary Medicine, Division of Applied Veterinary Research, Laurel, MD, 20708, United States, michael.myers@fda.hhs.gov Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Michael J Myers Christine M Deaver Elzabeth A Kenyon Sharla M Peters Heidi L Swaim Katyln Tanke Haile F Yancy Google Michael J Myers Christine M Deaver Elzabeth A Kenyon Sharla M Peters Heidi L Swaim Katyln Tanke Haile F Yancy Google Scholar Michael J Myers Christine M Deaver Elzabeth A Kenyon Sharla M Peters Heidi L Swaim Katyln Tanke Haile F Yancy PubMed Michael J Myers Christine M Deaver Elzabeth A Kenyon Sharla M Peters Heidi L Swaim Katyln Tanke Haile F Yancy Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.