Abstract
Dermal fibroblasts are the main actor in many proteins’ secretion, including collagen, preserving skin function. Free radicals are involved in skin aging and damages involving different cellular components. The imbalance between reactive oxygen species (ROS) amount and natural antioxidant enzymes negatively affects skin homeostasis. Natural compounds have recently emerged as a potential anti-aging tool in tissue regeneration. In the present paper we evaluated the antioxidant activity of white and red wines, considering their probable use, as raw materials, for the formulation of cosmetic products with anti-aging properties. We studied a method that would allow the removal of the alcoholic fraction of wines and determined their composition by LC-MS analysis. We then tested the possible cytotoxic effects of red and white wines on fibroblasts by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay, and their antioxidant activity by the catalase activity test in stressing conditions. Finally, we evaluated their anti-aging potential through the β-galactosidase colorimetric assay. Our results showed that wine extracts exhibit a remarkable antioxidant and anti-aging activity, especially on cells exposed to a marked stressful event. These properties could suggest their possible application as cosmetical products for skin regeneration.
Highlights
Introduction iationsThe skin is the most extensive organ of the body and has a multiplicity of functions, protecting the underlying tissues from chemical and mechanical insults, UV radiation, free radicals, and infections
In order to detect any deterioration of the polyphenols contained in the wine during the evaporation step, the samples before and after the treatment were subjected to the liquid chromatography analysis
The well-known particular, the well-known preventive effect of atherosclerosis depends on the antioxidant preventive effect of atherosclerosis depends on the antioxidant activity on LDL cholesterol, activity on LDL cholesterol, whose oxidation would lead to the capture by white blood whose oxidation would lead to the capture by white blood cells followed by the formation cells followed by the formation of atheromatous plaque[31,32]
Summary
The skin is the most extensive organ of the body and has a multiplicity of functions, protecting the underlying tissues from chemical and mechanical insults, UV radiation, free radicals, and infections. It plays a role in thermoregulation, has endocrine and biochemical functions, and is the organ of application and/or absorption of xenobiotics (drugs, poisons, cosmetics) [1,2,3]. Hormones, and the damaging effects of ultraviolet radiation can reduce the thickness and elasticity of the dermis, resulting in wrinkles and loss of skin tone [4,5]. The reactive oxygen species (ROS), resulting from the loss of electrons during aerobic metabolism or after exposure to environmental factors, are unstable species able to damage different
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