Identifying a novel powdery mildew resistance gene in a barley landrace from Morocco

Urszula Piechota,Jerzy H Czembor,Paweł C Czembor,Piotr Słowacki

doi:10.1007/s13353-019-00505-y

Abstract

Powdery mildew is a barley foliar disease that causes great loss in yield. Because of the limited number of effective resistance genes, efforts to identify new sources of resistance are frequently focused on genetically diversified landraces. The goal of this study was to characterise the powdery mildew resistance gene in barley line 2553-3 selected from the Moroccan landrace. Phytopathological testing against a set of differential pathogen isolates revealed different pattern responses of this gene from those of other known resistance genes. F2 and F2:3 (2553-3 × Manchuria) mapping populations were employed to investigate resistance inheritance. Two approaches were applied for the linkage analysis: in the first approach, 22 resistant and 21 susceptible homozygous F2 plants genotyped by the DArTseq platform (Diversity Arrays Technology, Pty. Ltd.) were used; in the second, 94 F2 plants were genotyped by converted DArTseq markers and SSRs. Both analyses delineated a new resistance gene on the short arm of chromosome 2H. The authors propose MlMor as a gene symbol for newly characterized powdery mildew resistance genes in barley line 255-3-3. The results presented herein provide a good foundation for the development of closer linkage markers and MAS breeding.

Highlights

Barley (Hordeum vulgare L.) is the second most important cereal according to harvest area in Europe (FAOSTAT 2016.http://www.fao.org/faostat) and is generally grown as fodder and for brewing purposes
The results presented provide a good foundation for the development of closer linkage markers and Keywords Barley (Hordeum vulgare L.)
The aim of the present study was to characterise a new source of barley resistance to powdery mildew

Summary

Introduction

Barley (Hordeum vulgare L.) is the second most important cereal according to harvest area in Europe Http://www.fao.org/faostat) and is generally grown as fodder and for brewing purposes. Barley has low environmental requirements and can be farmed under harsh conditions (Nevo 1992; Stanca et al 1992; Newton et al.2010; Honsdorf et al 2014), fungal infections are a cause of concern. Blumeria graminis (D.C.) Golovin ex Speer f. Hordei Em. Marchal (Bgh) is an obligate Ascomycota pathogen that causes powdery mildew, one of the most widespread foliar diseases. Marchal (Bgh) is an obligate Ascomycota pathogen that causes powdery mildew, one of the most widespread foliar diseases Infection by this fungus leads to yield loss and decreased feed and malting quality.

Objectives

Methods

Conclusion