Abstract
BMPR-1B gene closely related to the rate of ovulation and follicular maturation. In poultry, BMPR-1B gene is located on chromosome 14th consists of 13 exons. BMPR-1B was expressed in the granulosa cells of the ovary and teca interna poultry. BMPR-1B was able to accelerate the maturation of the follicles so that the ova produced increasingly. BMPR-1B gene diversity was associated with poultry production. DNA isolation was performed using GeneJET Genomic DNA Purification Kit from blood samples derived from 28 native chickens, 20 layer chickens and 10 arab layer chickens. A total of 58 DNA were used as a template in the process of BMPR-IB gene amplification using Polymerase Chain Reaction method. Primers used in this study according to Zhang et al. (2008), Forward primer 5 'ATG GCT GGG AAG TCT GGA TG 3'dan Reverse primer 5' TGC CTT CTG TGT TAA CCG C 3 'with a length of 581 bp. This primary amplify BMPR-1B gene segment from exon 6 to exon 7 including the entire intron 6 with an annealing temperature of 58 0 C. BMPR-IB gene diversity was detected using restriction enzymes HindIII were incubated for 2 hours. The results of the study was showed that Hind III restriction enzymes do not cut the BMPR-1B gene segments in the population of native chickens, arab layer chickens and layer chickens, they were monomorphic.
Highlights
Beberapa jenis ayam petelur yang umum dipelihara di Provinsi Riau adalah ayam ras petelur, ayam arab dan ayam kampung dengan tingkat produktivitas yang relatif masih rendah
BMPR-1B gene closely related to the rate of ovulation
BMPR-1B gene is located on chromosome 14th consists of 13 exons
Summary
Penelitian telah dilaksanakan pada Bulan Juli sampai dengan Desember 2015 di Laboratorium Pemuliaan dan Genetika Fakultas Pertanian dan Peternakan UIN Suska Riau. Materi yang digunakan adalah sampel darah dari populasi ayam arab (n= 20 ekor), ayam kampung (n=28 ekor) dan ayam ras petelur (n=10). Bahan dan alat yang digunakan untuk pengambilan sampel darah adalah spuit 3 ml, tabung vacutainer dengan EDTA 3 ml, ethanol absolut (96%), kapas beralkohol dan cool box. Penyimpanan sampel darah ayam kampung dan ayam ras petelur dilakukan pada tabung vacutainer dengan EDTA sedangkan ayam arab dengan penambahan alcohol absolut dengan perbandingan 1:1. Bahan-bahan yang digunakan untuk uji kualitatif DNA hasil isolasi adalah gel agarose 1% (0,5 g agarose + 50 ml dalam 1 x TAE, ethidium bromide, loading dye, marker (100 bp) dan Dilution Water.
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