IDENTIFIKASI E. COLI O157:H7, SALMONELLA SP, DAN SENSITIFITAS ANTIBIOTIKA DARI SUSU KAMBING DAN PRODUK OLAHANNYA

Abstract

<p>Susu kambing dan produk olahannya dapat terkontaminasi <em>E. coli </em>O157:H7 dan <em>Salmonella </em>sp. Tujuan penelitian ini untuk isolasi dan identifikasi <em>E. coli </em>O157:H7, <em>Salmonella </em>sp, dan sensitifitasnya terhadap antibiotika dari susu kambing dan produk olahannya. Sebanyak 15 sampel susu kambing dan produk olahannya seperti susu bubuk, permen, es krim, yogurt, dan krupuk masing-masing sebanyak 10, 3, 6, 4, dan 3 sampel. Semua sampel diperiksa terhadap <em>E. coli </em>O157:H7, dan <em>Salmonella </em>sp berdasarkan reaksi biokimia. Jumlah <em>E. coli </em>pada semua sampel dihitung dengan most probable number (MPN), sedangkan sensitifitas terhadap antibiotika dengan agar difusi. Hasil penelitian menunjukkan bahwa susu kambing dan produk olahannya tidak ditemukan <em>E. coli </em>O157:H7 dan <em>Salmonella </em>sp., tetapi <em>E. coli </em>non O157:H7 ditemukan pada susu kambing sebanyak 3/15 (20%) dengan jumlah >6 MPN/100ml. Semua produk olahan susu kambing memiliki jumlah <em>E. coli </em><3 MPN/100ml. <em>E. coli </em>dari susu kambing resisten terhadap cefiksime, kanamisin, tetrasiklin, sulfonamide, dan oksitetrasiklin masing-masing sebanyak 1/3 (30%), sedangkan ampisilin dan amoksilin 100%.</p><p> </p><p><strong>Goat milk and dairy products could be contaminated with <em>E. coli </em>O157:H7 and <em>Salmonella </em>sp</strong>.</p><p>The purpose of this study was to isolation and identification of <em>E. coli </em>O157:H7, <em>Salmonella </em>sp, and antibiotic sensitivity from goat milk and dairy products. A total of 15 samples from goat milk and dairy products such as milk powder, candy, ice cream, yogurt, and crackers respectively 10, 3, 6, 4, and 3 samples. All samples were analyzed for <em>E. coli </em>O157: H7, and Salmonella sp with biochemical reaction. Total of <em>E. coli </em>in all samples was measured with most probable number (MPN) and antibiotic sensitivity with diffusion agar. These study showed that goat milk and dairy products not found <em>E. coli </em>O157:H7 and <em>Salmonella </em>sp., whereas <em>E. coli </em>non O157:H7 was found in goat milk 3/15 (20%) with total <em>E. coli </em>>6 MPN/100ml. All dairy goat products have total <em>E. coli </em><3 MPN/100ml. <em>E. coli </em>from goat milk was resistant to cefixime, kanamycin, tetracycline, sulfonamide, and oxytetracycline 1/3 (30%) respectively, but ampicillin and amoxicillin 100%.</p>