Abstract
BackgroundCoarctation of the aorta (CoA), presenting with local stenosis of the aorta is involved in many cardiovascular processes. However, there has been little research on the mechanism of coarctation of the aorta.MethodsAltered proteins were identified by isobaric tag for relative and absolute quantitation (iTRAQ) technology in 8 participants, and further analysed by heatmap, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) and Search Tool for the Retrieval of Interacting Gene (STRING). Of these, two vascular structure-related proteins were further validated by using enzyme-linked immunosorbent assay (ELISA) in a new cohort of CoA patients.Results39 differentially expressed plasma proteins were first identified in patients with coarctation of the aorta by iTRAQ. Of these, fibulin-1 (FBLN1) and insulin-like growth factor-binding protein complex acid labile subunit (ALS) were considered candidates and further validation also showed that the level of FBLN1 in the CoA group (8.92 ± 2.36 μg/ml) was significantly higher compared with control group (6.13 ± 1.94 μg/ml), and the level of ALS in CoA children (348.08 ± 216.74 ng/ml) was significantly lower than the level in normal children (619.46 ± 274.08 ng/ml).ConclusionsThe differentially expressed proteins identified in the plasma from CoA patients indicated that they may play critical roles in CoA and that they could potentially be utilized as biomarkers for diagnosis. Altered vascular related proteins were associated with COA. These results provide a foundation for further understanding and studying the aetiology and pathogenesis of coarctation of the aorta.
Highlights
Coarctation of the aorta (CoA) is a diffuse arteriopathy, shown as a local stenosis of the aortic lumen on account of medial wall in-folding and thickening of aortic wall tissue [1]
We identified two differentially expressed proteins (DEPs) related to vascular structure from the plasma of CoA children compared with those in the plasma of normal children by using the isobaric tag for relative and absolute quantitation (iTRAQ) method
Details of all 39 DEPs have been provided in Supplemental Materials Table 2
Summary
Coarctation of the aorta (CoA) is a diffuse arteriopathy, shown as a local stenosis of the aortic lumen on account of medial wall in-folding and thickening of aortic wall tissue [1]. The influence of CoA in the cardiovascular system manifested as differences in the blood pressure of. That CoA causes abnormalities in anatomy, and causes changes in pathology and physiology [3,4,5,6]. CoA influenced the aortic arch and led to wider vasculopathy [7], such as aortic rupture or dissection, adolescent hypertension, coronary and cerebral artery diseases [8, 9]. Coarctation of the aorta (CoA), presenting with local stenosis of the aorta is involved in many cardiovascular processes. There has been little research on the mechanism of coarctation of the aorta
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