Identification ofTaxus cuspidata sieb. et Zucc. endophytic fungi-new species, species known and their metabolite

Abstract

A total of 94 isolates of endophytic fungi were isolated from the bark of 200-yr.-oldTaxus cuspidata Sieb. et Zucc. In the primeval forest of the Changbai Mountain Natural Reserve, and 19 species of endophytic fungi were identified, including 10 new recorded-genus-species, 2 new species (Phomopsis longiscoleosporu Y. Xiang et Lu An Guo Wu Wen Fang,Coniothyrium macrospoum Y. Xiang J.X. et Lu An Guo Wu Wen Fang), 1 new varied species (Altermaria alternata (Fr.) Keissler var.taxi Y. Xiang et Lu An Guo) and 6 known species of China (Eurotium amstelodomi Mgngin,Eurotium repens de Bary,Botrytis sp.,Penicillium citrinum Thom,Epicoccum nigrium LinK,Fusarium sp.). Through thin layer chromatography (TLC), liquid fermentation metabolite of the strains was determined, and four strains (Alternaria altemata (Fr.) Keissler var.taxi Y. Xiang et Lu An Guo Wu Wen Fang,Botrytis sp.,Eurotium amsteloodomi Mgngin,Eurotium repens de Bary) were screened out, whose metabolites reacted positively with the vanillic aldehyde that was one special taxoid developer. Among the four strains,Altermaria altermata (Fr.) Keissler var.taxi Y. xiang et Lu An Guo, produced one compound largely, which positively reacted with one alkaloids developer— Bismuth potassium iodide. The compound is identified as taxoids type through spectrum analysis. This demonstrates thatAlternaria alternata (Fr.) Keissler var.taxi Y. Xiang et Lu An Guo can highly produce taxoids largely.