Abstract
AbstractMariner-like element sequences were recovered from the genome of the orange wheat midge,Sitodiplosis mosellana(Géhin), with degenerate PCR primers designed to conserved regions ofmarinertransposases. The deduced amino acid sequences of themariner-like transposases fromS. mosellanashowed 67% to 78% identity with the peptide sequences of othermarinertransposases. A phylogenetic analysis revealed that themariner-like elements fromS. mosellanagrouped in the mauritiana subfamily ofmarinertransposons. Results from Southern blot analysis suggestmariner-like elements are at a moderate copy number in the genome ofS. mosellana.
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