Identification of XbaI polymorphism of the estrogen receptor alpha in mexicans at early posmenopause

Abstract

INTRODUCTIONMenopause is the cessation of menstrual cycle, is diagnosed after at least twelve consecutive months whitout bleeding, it occurs due to the reduced functioning of the ovaries, resulting in lower levels of ovarian hormones primarily estradiol (NAMS, 2012). Estrogen suppression has been correlated with higher incidence of cardiovascular and metabolic diseases observed in this age group.Estrogens exert its actions through their receptors, the most studied isoforms of these receptors are: the alpha (ER‐α), beta (Er‐β) and the a G coupled (GPR30). Due to its action and location, an important role in the preservation of cardiovascular and metabolic health has been attributed to ER‐ α (Raushember y Cols, 2016).Several ER‐α polymorphisms have been described, one of the most interesting due to its association with the etiology of cardiovascular pathologies, is the termed as XbaI (rs9340799A> G). This one, consists in the change of an adenine for a guanine, which modifies the affinity of the receptor for its ligand. The X allele has been linked to increased risk of bone demineralization, dyslipidemia, high body mass index (BMI), and oncologic diseases, such as breast cancer and ovarian tumors (chedrahui y Cols,2014; Lobo y Cols, 2014).The aim of this study was to identify the genotype of XbaI polymorphism in mexican posmenopausal women.MATERIALS AND METHODSA descriptive, observational, prospective, transversal, cohort study was carried out in 87 early posmenopausal women who attend to the climacteric clinic of “Hospital general Ignacio Zaragoza”. Clinical data, hormonal profile, blood pressure, antropometric parameters were evaluated. XbaI polymorphism was identified by restriction fragment length polymorphism (RFPL) method.RESULTSThe mean age was 53.01±5.08 years, the BMI average was 28.77±3.88 m/kg2. The averages of systolic, diastolic, and mean blood pressure were 116±10.44 mmHg, 78.7±9.39mmHg y 91.58±9.1 mmHg, respectively;27 patients had hypertension diagnostic under pharmacological treatment.We found 58.87%, 29.89% and 19.24% for xx, Xx and XX genotypes respectively (Graph 1).The averages of systolic, diastolic, mean blood pressure and BMI according XbaI genotype are showed in table 1, there was no statistical differences between groups.CONCLUSIONThe most common genotype in mexican posmenopausal women was xx.There was no differences in blood pressure and BMI according genotype.All patients had elevated values for BMI, independently of her XbaI genotypesSupport or Funding InformationnoneThis abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.