Abstract
BackgroundMicroRNAs (miRNAs) play important regulatory roles in development and stress response in plants. Wild soybean (Glycine soja) has undergone long-term natural selection and may have evolved special mechanisms to survive stress conditions as a result. However, little information about miRNAs especially miRNAs responsive to aluminum (Al) stress is available in wild soybean.ResultsTwo small RNA libraries and two degradome libraries were constructed from the roots of Al-treated and Al-free G. soja seedlings. For miRNA identification, a total of 7,287,655 and 7,035,914 clean reads in Al-treated and Al-free small RNAs libraries, respectively, were generated, and 97 known miRNAs and 31 novel miRNAs were identified. In addition, 49 p3 or p5 strands of known miRNAs were found. Among all the identified miRNAs, the expressions of 30 miRNAs were responsive to Al stress. Through degradome sequencing, 86 genes were identified as targets of the known miRNAs and five genes were found to be the targets of the novel miRNAs obtained in this study. Gene ontology (GO) annotations of target transcripts indicated that 52 target genes cleaved by conserved miRNA families might play roles in the regulation of transcription. Additionally, some genes, such as those for the auxin response factor (ARF), domain-containing disease resistance protein (NB-ARC), leucine-rich repeat and toll/interleukin-1 receptor-like protein (LRR-TIR) domain protein, cation transporting ATPase, Myb transcription factors, and the no apical meristem (NAM) protein, that are known to be responsive to stress, were found to be cleaved under Al stress conditions.ConclusionsA number of miRNAs and their targets were detected in wild soybean. Some of them that were responsive to biotic and abiotic stresses were regulated by Al stress. These findings provide valuable information to understand the function of miRNAs in Al tolerance.
Highlights
IntroductionMicroRNAs (miRNAs) play important regulatory roles in development and stress response in plants
MicroRNAs play important regulatory roles in development and stress response in plants
Deep-sequencing results of wild soybean To identify miRNAs responsive to Al stress, two small RNA libraries constructed from the roots of Al-treated and Al-free wild soybean seedlings were sequenced on the Illumina Genome Analyzer IIx
Summary
MicroRNAs (miRNAs) play important regulatory roles in development and stress response in plants. Duressa and coworkers [23] used DNA microarray technology to identify putative genes in the Al-tolerant soybean line PI 416937 and reported that many genes involved in transcription activation, stress response, cell metabolism and signaling were differentially expressed in Al-tolerant soybean. They concluded that Cys2His and ADR6 transcription activators, cell wall modifying enzymes, and phytosulfokine growth factors might play roles in soybean Al tolerance [23]
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