Abstract
In this research, to identify natural wild hosts of TYLCV, 44 symptomatic samples were collected in or around harvested tomato fields in south-eastern Iran and tested for the TYLCV infection by PCR. Among four PCR-positive plant species, full-length genome of TYLCV was amplified by rolling circle amplification (RCA) method only from a ground cherry (Physalis divaricata L., Solanaceae) sample. Cloning and full-length genome sequencing of a ground cherry isolate (G4) showed that it comprises 2756 nucleotides (nts) in length and shares 87.7%–95.2% nt sequence identities with TYLCV isolates reported from Iran, Oman and Pakistan. The G4 isolate of TYLCV was successfully transmitted to tomato plants and the original host (P. divaricata) via agroinoculation and showed typical symptoms. According to the results of this research, four symptomatic weed species appear to be alternative hosts of TYLCV and play the role of the primary inoculum for the viral infection.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
