Identification of wheat and tritordeum chromosomes by genomic in situ hybridization using total Hordeum chilense DNA as probe

Abstract

Total genomic Hordeum chilense DNA probe was hybridized to somatic chromosome spreads of Triticum aestivum 'Chinese Spring' and to four advanced tritordeum lines, the latter being the fertile amphiploid between H. chilense and durum wheat (2n = 6x = 42, AABBH(ch)H(ch)). The probe hybridized strongly to the B-genome chromosomes and to one or two bands on the A-genome chromosomes present in both wheat and tritordeum alloploids. Bands on chromosomes 1D, 2D, and 7D from hexaploid wheat were also detected. Genomic H. chilense DNA probe identified 16 chromosome pairs of the chromosome complement of hexaploid wheat and all A- and B-genome chromosomes present in the tritordeum amphiploids. The in situ hybridization patterns observed correspond to those previously reported in wheat by both N-banding and in situ hybridization with the GAA-satellite sequence (Pedersen and Langridge 1997), allowing the identification of these chromosomes. Variation among the tritordeum amphiploids for hybridization sites on chromosomes 2A, 4A, 6A, 7A, 4B, 5B, and 7B was observed. Despite of this polymorphism, all lines shared the general banding pattern. When used as probe, total H. chilense genomic DNA labeled the H. chilense chromosomes over their lengths allowing the identification of 14 H. chilense chromosomes present in the tritordeum amphiploids. In addition, chromosome-specific telomeric, interstial, and centromeric hybridization sites were observed. These hybridization sites coincide with N-banded regions in H. chilense allowing the identification of the individual H. chilense chromosomes in one of the amphiploid. The N-banded karyotypes of H. chilense (accessions H1 and H7) are presented.