Identification of virulence genes in Pseudomonas aeruginosa isolated from clinical specimens in Ad- Diwaniya Teaching Hospital.

Abstract

The present study was carried outto verify the role of virulence genes in establishment of opportunistic infections of the P. aeruginosa by using the PCR technique. A total of 50 swabs were collected from wound,burn and sputum samples of patients who admitted at Ad-Diwaniya province during the period from May – October 2017. Brain Heart Infusion broth and chrome agar media were used for isolation of P. aeruginosa. Bacterial genomic DNA was extracted from P.aeruginosa isolates for detection of the virulence genes (exoS,toxA,and oprI) by using PCR technique. The isolation percent of P. aeruginosa was high (78.26%) in burn infections rather than wound infections and pulmonary tract. The burn infection P. aeruginosaisolatesshowed high occurrence of virulence genes more than ordinary wound infections isolates as well as the virulence gene (ToxA) was highlypresent in most isolates. It wasconcluded that the expressionof virulence genes in Pseudomonas aeruginosais important factor in causinghuman infections especially (ToxA) gene and the PCR technique is ahighly specific and accurate technique as a rapid diagnostic laboratory method for Pseudomonas aeruginosa.