Abstract
Trueperella (T.) bernardiae is a well-known bacterial pathogen in infections of humans, rarely in animals. In the present study, five T. bernardiae isolates, isolated from five Peking ducks of four different farms, were identified by phenotypic properties, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, and genotypically by sequencing the 16S ribosomal RNA (rRNA) gene, the superoxide dismutase A encoding gene sodA, and the glyceraldehyde-3-phosphate dehydrogenase encoding gene gap. In addition, the T. bernardiae isolates could be identified with a newly developed loop-mediated isothermal amplification (LAMP) assay based on the gyrase encoding housekeeping gene gyrA. All these tests clearly identified the T. bernardiae isolates to the species level. However, the detection of the specific gene gyrA with the newly designed LAMP assay appeared with a high sensitivity and specificity, and could help to identify this bacterial species in human and animal infections in future. The importance of the T. bernardiae isolates for the clinical condition of the ducks and for the problems at farm level remains unclear.
Highlights
Trueperella (T.) bernardiae is a gram-positive, non-motile, and facultatively anaerobic coccobacillus which was originally identified as a coryneform group 2 bacterium (Na’Was et al 1987)
T. bernardiae was first described as an opportunistic pathogen and later could be identified alone or in combination with other bacteria as causing joint infections (Gilarranz et al 2016; Gowe et al 2018), urinary tract infections (Lepargneur et al 1998), abscesses (Parha et al 2015; VanGorder et al 2016; Calatrava et al 2019; Pan et al 2019), wound infections (Weitzel et al 2011; Rattes et al 2016; Cobo et al 2017), a diabetic foot infection (Schneider et al 2015), bacteremia (Otto et al 2013; Roh et al 2019), and septic thrombophlebitis (Lawrence et al 2018)
Five T. bernardiae isolates recovered from post mortem samples of Peking ducks (Anas platyrhynchos domesticus) could be identified phenotypically and genotypically, and with a newly described loopmediated isothermal amplification (LAMP) assay based on the housekeeping gene gyrA
Summary
Trueperella (T.) bernardiae is a gram-positive, non-motile, and facultatively anaerobic coccobacillus which was originally identified as a coryneform group 2 bacterium (Na’Was et al 1987). Five T. bernardiae isolates recovered from post mortem samples of Peking ducks (Anas platyrhynchos domesticus) could be identified phenotypically and genotypically, and with a newly described loopmediated isothermal amplification (LAMP) assay based on the housekeeping gene gyrA.
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