Identification of TRPM6 and TRPM7 expression changes in response to a diet supplemented with inulin in porcine kidney

Abstract

Abstract. Magnesium is the fourth most abundant mineral element in vertebrates and the second most common intracellular cation. Recently identified Mg2+-specific channels – TRPM6 and TRPM7 – have been shown to be essential for whole-body and cellular Mg2+ homeostasis. The aim of the study was to determine the effect of inulin on the expression of TRPM6 and TRPM7 in the renal cortex and medulla of growing pigs. The study was carried out on 16 Danbred × Duroc castrated male piglets fed a cereal-based diet without inulin or with 2 % addition of inulin from chicory root from the 10th day of life. In pigs fed a diet with inulin, TRPM6 expression was greater in both the renal cortex and medulla compared to the control group. The expression of TRPM7 in both the renal cortex and medulla in the control group and in piglets fed a diet enriched with inulin was relatively stable. To our knowledge, this is the first study aimed at the identification of TRPM6 and TRPM7 in the kidneys of pig. It is proposed that inulin addition to fodder resulted not only in a magnesium absorption increase, but also, due to prolonged low plasma Mg concentration of examined piglets, renal magnesium retention. Therefore, higher magnesium reabsorption via increased TRPM6 expression in the kidney was probably observed in order to supplement deficiencies of this element. Diet-unresponsive expression of TRPM7 supports the concept that this channel is not involved in the extracellular magnesium homeostasis.