Abstract
Antrodia camphorata Wu, Ryvarden & Chang (Polyporaceae) is an endemic parastic fungus in Taiwan. It grows slowly on the heartwood of Cinnamomum kanehirai and produced orange-red color fruiting body on the surface of wood. The fruiting body of A. camphorata has been used as antidote for food or drug intoxication and treatment for hepatoma. The extract or compounds isolated from fruiting body or mycelium of A. campharata showed immuno-enhance, neuroprotective, hepatoprotective, cytotoxicity on tumor cells, anti-inflammatory, and antioxidative, activities. The bioactive components of A. camphorata were identified as polysaccharides, triterpenoids, steroids, bezenoids, and maleic acd/succinic acid derivatives. High-performance liquid chromatography-photodiode array detector-electrospray ionization mass spectrometry (HPLC-PAD-ESI/MS) was developed to characterize the triterpenoids in A. camphorata mycelium and fruiting body. Zhankuic acid D, dehydroeburicoic acid (or antcin B), dehydroesulfurenic acid, zhankuic acid, antcin C, and antcin A were identified with selected ion monitoring (SIM) of 481, 467, 483, 485, 469, and 453 amu ions from ethanol extract of A. camphorata fruiting body. Antcin C, antcin A, zhankuic acid C, dehydresulfurinic acid, and dehydroeburicoic acid (or antcin B) were identified with SIM of 469, 453, 485, 483, and 467 amu ions from ethanol extract of A. camphorata mycelium. This HPLC-PAD-ESI/MS is suitable to identify the triterpenoids in the commercial products of A. camphorata mycelium or fruiting body.
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