Abstract
BackgroundThis study aimed to identify the transcription factors of nitrate reductase genes (NIA1 and NIA2) promoters and hypothetical cis-element of NRE2. Based on the constructed cDNA library of Nicotiana tabacum K326, a yeast one-hybrid system was established using the Matchmaker® Gold Yeast One-Hybrid Library Screening System from Clontech. The transcription factors of NIA1 andNIA2 promoters and NRE2 cis-elements were screened.ResultsAfter sequencing and bioinformatics analysis, 15 cDNA sequences were identified: 9 for NIA1 (including XP_016503563.1 and NP_001312236.1), 3 for NIA2 (including XP_016510250.1), and 3 for NRE2 (including XM_016576899.1). XP_016503563.1 was annotated in PREDICTED: CRM-domain containing factor CFM3, and NP_001312236.1chloroplastic/mitochondrial-like in Nicotiana tabacum. NP_001312236.1 was annotated in Sulfite oxidase-like of Nicotiana tabacum. XP_016510250.1 was annotated as PREDICTED: uncharacterized protein LOC107827596 in Nicotiana tabacum. XM_016576899.1 was annotated in PREDICTED: Nicotiana tabacum RING-H2 finger protein ATL16-like (LOC107759033).ConclusionA yeast one-hybrid library was successfully constructed. The identified transcription factors may provide a theoretical basis for the study of plant nitrate reductase.
Highlights
This study aimed to identify the transcription factors of nitrate reductase genes (NIA1 and NIA2) promoters and hypothetical cis-element of NRE2
Our present study aimed to investigate the role of NRE2 in NIA1 and NIA2 promoters and to identify the transcription factor (TF) binding to NIA1, NIA2, and NRE2 based on a yeast one-hybrid system
In order verify whether the change of NRE2 elements could influence the expression of NIA1 and NIA2 genes, we added four NRE2 in NIA1 and NIA2 promoters
Summary
This study aimed to identify the transcription factors of nitrate reductase genes (NIA1 and NIA2) promoters and hypothetical cis-element of NRE2. The transcription factors of NIA1 andNIA2 promoters and NRE2 cis-elements were screened. Nitrogen is quantitatively the most important nutrient that plants acquire from soil [1]. Nitrogen deficiency exerts severe pressure on plant growth, development, and photosynthesis, limiting plant productivity [2,3,4]. Nitrate is a primary nitrogen source for most plants [5]. It functions as a signal molecule that promptly triggers changes in metabolism, growth, and gene expression in plants [6, 7]. Overexpression of NR and NIR genes has an influence
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