Identification of Three Egyptian Annona Cultivars Morhologically and Biochimically using Rapd Analysis

Abstract

To detect remarkable fingerprinting and evaluate genetic variation and similarity among three Egyptian cultivarsAnnona sp. (which cultured in Sabahia Horticulture Research Station, during 2016 and 2017 seasons atSabahiaHorticulture Research Station (HRI), namely: Abd Elrazek, Baladi, and Senigalinsis) first morphologically using Leafdiameter, then seed number and fruit dimension, it was found that fruit and seeds could be used to evaluate variation andgenetic similarity among cultivars. Where Abdelrazik was the lowest in seed number with 37 seeds comparing with Baladiand Sengalinsis with 73 and 127 respectively.Moreover, fruit could be used to evaluate genetic similarity among cultivars. Abdelrzak was the lowest in seednumber with 37 seeds comparing with Baladi and Senigalinses with 73 and 127 respectively.Leaf parameters were used as a species and varieties diversity and found useful with longest leaf length forAbdelrzak and Sengalinsis 13.5 cm and 12.43 cm respectively. While Baladi was the shortest with 0.30 cm.`Finally molecular marker techniques (Random Amplified polymorphic DNA (RAPD) fingerprinting technique,)were used Different polymorphism percentages were recorded for four random primers (OPG1, POG2, OPG3 andOPG4) through this investigation. Generally, all genotypes could be distinguished via four random primers understudy. First primer reflected high polymorphic percentage with 82 % of polymorphism. On the other hand,high similarity percentage and lowest polymorphism percentage were recorded for fourth primer with 50 % ofpolymorphism.,,, A dendrogram was done to depict the pattern of relationships between the studied cultivars andtheir genetic diversity Not only it was fairly good But Also it reveal the need of further studies with new suitabletechniques.