Abstract
Ribosome-inactivating proteins (RIP) are RNA- N-glycosidases widely diffused in plants which depurinate ribosomal RNA at a specific universally conserved position, A4324 in rat ribosomes. A small group of RIPs (cofactor-dependent RIPs) require ATP and tRNA to reach maximal activity on isolated ribosomes. The tRNA which stimulates gelonin was identified as tRNA Trp. The present paper reports the identification of three other tRNAs which stimulate agrostin (tRNA Ala), barley RIP (tRNA Ala, tRNA Val) and PAP-S (tRNA Gly), while for tritin-S no particular stimulating tRNA emerged. The sequences of tRNA Val and tRNA Gly correspond to the already known ones (rabbit and man, respectively). The tRNA Ala (anticodon IGC) identifies a new isoacceptor. Only the stimulating activity of the tRNA Ala for agrostin approaches the specificity previously observed for the couple gelonin-tRNA Trp.
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