Abstract
The sterol compositions of mutants of the yeasts ofSaccharomyces cerevisiae andCandida guilliermondii have been characterized by the methods of GLC, TLC, UV spectroscopy, and chromato-mass spectrometry. The possibility has been shown of identifying yeast sterols on the basis of a comparison of the intensities of the peaks of the fragmentary ions having a common nature for all sterols (m/z≧200). A change in the sterol composition in mutant yeasts as compared with the initial strain has been detected which is obviously connected with the blockage of the routes for the biosynthesis of ergosterol in the yeast cell.
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