Identification of the soybean HyPRP family and specific gene response to Asian soybean rust disease.

Lauro Bücker Neto,Caroline Cabreira,Ricardo Luís Mayer Weber,Rafael Rodrigues De Oliveira,Luciane Maria Pereira Passaglia,Ricardo Vilela Abdelnoor,Francismar Correa Marcelino,Maria Helena Bodanese Zanettini,Marta Bencke,Beatriz Wiebke-Strohm

doi:10.1590/s1415-47572013005000017

Lauro Bücker Neto, Caroline Cabreira + Show 8 more

Open Access

https://doi.org/10.1590/s1415-47572013005000017

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Abstract

Soybean [Glycine max (L.) Merril], one of the most important crop species in the world, is very susceptible to abiotic and biotic stress. Soybean plants have developed a variety of molecular mechanisms that help them survive stressful conditions. Hybrid proline-rich proteins (HyPRPs) constitute a family of cell-wall proteins with a variable N-terminal domain and conserved C-terminal domain that is phylogenetically related to non-specific lipid transfer proteins. Members of the HyPRP family are involved in basic cellular processes and their expression and activity are modulated by environmental factors. In this study, microarray analysis and real time RT-qPCR were used to identify putative HyPRP genes in the soybean genome and to assess their expression in different plant tissues. Some of the genes were also analyzed by time-course real time RT-qPCR in response to infection by Phakopsora pachyrhizi, the causal agent of Asian soybean rust disease. Our findings indicate that the time of induction of a defense pathway is crucial in triggering the soybean resistance response to P. pachyrhizi. This is the first study to identify the soybean HyPRP group B family and to analyze disease-responsive GmHyPRP during infection by P. pachyrhizi.

Highlights

Soybean [Glycine max (L.) Merril], one of the most important and extensively cultivated crops in the world, is widely used for human and animal consumption because of the high protein and oil content of its seeds
We describe the identification and annotation of the soybean group B Hybrid proline-rich proteins (HyPRPs) family and its expression in different tissues based on microarray analysis
Sequences that shared the general organization of HyPRPs were aligned by their carboxy-terminal domain in order to evaluate the presence of the eight-cysteine motif; no gaps were inserted in the conserved 8CM core

Summary

Introduction

Soybean [Glycine max (L.) Merril], one of the most important and extensively cultivated crops in the world, is widely used for human and animal consumption because of the high protein and oil content of its seeds. Unfavorable field conditions may severely restrict the soybean yield, with one of the major concerns among Brazilian soybean producers being Asian soybean rust (ASR) disease. ASR, a severe disease caused by the fungus Phakopsora pachyrhizi, results in significant yield losses in soybean production and is rapidly spreading around the world (Pivonia et al, 2005; Carmona et al, 2005). Understanding the mechanisms that regulate the expression of stress-related genes is a fundamental issue in plant biology and is essential for the genetic improvement of soybean. As part of a study aimed at improving the abil-

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