Abstract
Despite the important role of MAPKs in signal transduction, their functions in the cellulase hyper-producing filamentous fungus Hypocrea jecorina haven't been studied except for the Hog1-like Tmk3. In this work, we constructed and explored the features of H. jecorina Δtmk2 to identify the role of this Slt2-homologous Tmk2. It is suggested from the results that Tmk2 is involved in cell wall integrity, sporulation and cellulase production. Although bearing similar roles in cell wall integrity maintenance, Tmk2 and Tmk3 appear to also have distinct functions: Tmk3 participates in high osmolarity resistance while Tmk2 does not; Tmk2 participates in sporulation but not Tmk3; Tmk3 is involved in promoting cellulase production while Tmk2 is involved in repressing cellulase formation. These studies provide the first insight into the function of Tmk2 in H. jecorina and contribute to understanding the signal transduction processes leading to the regulation of cellulase production in this important cellulase hyper-producer.
Highlights
Despite the important role of MAPKs in signal transduction, their functions in the cellulase hyper-producing filamentous fungus Hypocrea jecorina haven’t been studied except for the Hog1-like Tmk[3]
Bearing similar roles in cell wall integrity maintenance, Tmk[2] and Tmk[3] appear to have distinct functions: Tmk[3] participates in high osmolarity resistance while Tmk[2] does not; Tmk[2] participates in sporulation but not Tmk[3]; Tmk[3] is involved in promoting cellulase production while Tmk[2] is involved in repressing cellulase formation. These studies provide the first insight into the function of Tmk[2] in H. jecorina and contribute to understanding the signal transduction processes leading to the regulation of cellulase production in this important cellulase hyper-producer
The ratios of colony diameters between H. jecorina Dtmk[2] and parent strain didn’t decrease when NaCl concentrations increased: the ratios were respectively 0.37, 0.51, 0.62, 0.71, 0.79 and 0.81 for plates containing 0 M, 0.2 M, 0.4 M, 0.6 M, 0.8 M and 1 M NaCl. These results suggested there is no apparent increase of sensitivity to higher contents of NaCl following tmk[2] deletion, and further suggested Tmk[2] is not involved in combating high osmolarity
Summary
Despite the important role of MAPKs in signal transduction, their functions in the cellulase hyper-producing filamentous fungus Hypocrea jecorina haven’t been studied except for the Hog1-like Tmk[3]. Bearing similar roles in cell wall integrity maintenance, Tmk[2] and Tmk[3] appear to have distinct functions: Tmk[3] participates in high osmolarity resistance while Tmk[2] does not; Tmk[2] participates in sporulation but not Tmk[3]; Tmk[3] is involved in promoting cellulase production while Tmk[2] is involved in repressing cellulase formation These studies provide the first insight into the function of Tmk[2] in H. jecorina and contribute to understanding the signal transduction processes leading to the regulation of cellulase production in this important cellulase hyper-producer. The functions of Fus[3] homologues in filamentous fungi are quite diverse, out of which the participation in the secretion of extracellular proteins is of particular interest, exemplified in T. virens[33,34] and Trichoderma atroviride[35]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
