Identification of the risk of IHD development in young people, formation of a diagnostic model

Abstract

Background and Aims : To study the level of biomolecules characterizing the main pathogenetic links of coronary atherosclerosis. To develop a model for diagnosing the risk of coronary heart disease in young people based on significant biochemical parameters.Methods: The study included 49 people with IHD and 127 people without IHD. All participants signed an Informed Consent. A survey was conducted using validated questionnaires. Anamnestic data, anthropometry, functional examinations were collected from the all participants. Total antioxidant activity, MDA-oxLDL, SOD, MCP-1, TNF-alpha, IL-1, IL-6, IL-8, total cholesterol, TG, HDL and LDL were determined in blood serum using enzyme immunoassay, multiplex assay and enzymatic method.Results: The analysis of the data obtained revealed a difference in concentrations between the studied subgroups for the total antioxidant activity, SOD, MCP-1 and IL-6. In patients with IHD, the level of the total antioxidant activity was significantly lower by 2.9 times and MCP-1 by 1.4 times, the concentration of SOD and IL-6 was higher by 16 and 30%, respectively. For interpretation, we used a scatter diagram of the density of values - SHAP, reflecting the degree of influence of each parameter on the resulting variable. The greatest contributions to the model project were made by: the total antioxidant activity, SOD, MCP-1, IL-6 and HDL.Conclusions: The data obtained by us can be recommended for the development of a coronary heart disease risk meter in young people, which includes the most significant laboratory parameters. This study was conducted within the framework of the project RSF 21-15-00022. Background and Aims : To study the level of biomolecules characterizing the main pathogenetic links of coronary atherosclerosis. To develop a model for diagnosing the risk of coronary heart disease in young people based on significant biochemical parameters. Methods: The study included 49 people with IHD and 127 people without IHD. All participants signed an Informed Consent. A survey was conducted using validated questionnaires. Anamnestic data, anthropometry, functional examinations were collected from the all participants. Total antioxidant activity, MDA-oxLDL, SOD, MCP-1, TNF-alpha, IL-1, IL-6, IL-8, total cholesterol, TG, HDL and LDL were determined in blood serum using enzyme immunoassay, multiplex assay and enzymatic method. Results: The analysis of the data obtained revealed a difference in concentrations between the studied subgroups for the total antioxidant activity, SOD, MCP-1 and IL-6. In patients with IHD, the level of the total antioxidant activity was significantly lower by 2.9 times and MCP-1 by 1.4 times, the concentration of SOD and IL-6 was higher by 16 and 30%, respectively. For interpretation, we used a scatter diagram of the density of values - SHAP, reflecting the degree of influence of each parameter on the resulting variable. The greatest contributions to the model project were made by: the total antioxidant activity, SOD, MCP-1, IL-6 and HDL. Conclusions: The data obtained by us can be recommended for the development of a coronary heart disease risk meter in young people, which includes the most significant laboratory parameters. This study was conducted within the framework of the project RSF 21-15-00022.