Identification of the Properties and Function of the Unknown Protein with Accession Number AT2G15110.1 on the TAIR Website

Abstract

Background: Nowadays, with the help of genomics and proteomics, numerous genes and proteins have been discovered, however, the function and role of most of them are still unknown. Using bioinformatics tools can be a major step in the identification of the function of these genes and proteins. Methods: In this study, we applied various bioinformatics software to identify the unknown protein properties with the AT2G15110.1 accession number on the Arabidopsis Information Resource website. Operations, such as identification of general protein properties, blasting amino acid sequences, detection of motifs and domains in the sequence, examination of the second and third protein structures, exploration of ligands, assessment of proteins involved with the target protein as well as recognition of the target protein location in the cell, were carried out. Results: The results showed that the query protein had no significant homology in terms of sequence, three-dimensional structure, and any interaction with known proteins. Additionally, it was observed that the presence probability of this protein in the nucleus organelle was more than in other organelles, and it only has one domain of unknown function. Conclusions: The results of this study can be basic information for other researchers who seek to identify this unknown protein and determine the right pathway for the identification of mentioned protein function through bioinformatics tools or laboratory methods. Based on the results of this study, laboratory methods are recommended for subsequent studies.