Identification of synaptic pattern of kainate glutamate receptor subtypes on direction-selective retinal ganglion cells

Abstract

In this article we investigate the distributions of kainate glutamate receptor subtypes GluR5-7 and KA1, 2 on the dendritic arbors of direction-selective (DS) retinal ganglion cells (RGCs) of the rabbit retina to search for anisotropies, which might contribute to a directional preference of DS RGCs. The distribution of the kainate receptor subunits on the DS RGCs was determined using antibody immunocytochemistry. DS RGCs were injected with Lucifer yellow and the cells were identified by their characteristic morphology. The double-labeled images of dendrites and receptors were visualized using confocal microscopy and were reconstructed from high-resolution confocal images. We found no evidence of asymmetry in any of the kainate receptor subunits examined on the dendritic arbors of both On and Off layers of DS RGCs. Our results indicate that direction selectivity appears to lie in the neuronal circuitry afferent to the ganglion cell.