Abstract
Cercospora brachiata is a phytopathogenic fungus. To know more about the metabolites produced by this fungus, the objective of this work was to identify, isolate and characterize substances present in extracts of the growth broth and mycelium, using gas chromatography with mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR). It was also objective to evaluate the antibacterial activity of the extracts. Among the compounds identified, fatty acids, esters, and steroids can be highlighted. The main compounds identified are 9-hexadecenoic, hexadecenoic, oleic, octadecanoic, lauric, myristic, palmitic, doceno-13-enoic, stearic, linoleic, and nonadecanoic acids present in almost all extracts. For the antibacterial activity, the broth microdilution method was used. The ethyl acetate extract of the mycelium presented inhibitory concentrations (MICs) against the bacterium Actinomyces naeslundii (100 μg mL−1) and Streptococcus sanguinis (200 μg mL−1). Finally, two steroids were isolated and identified in the hexane extract of mycelium: ergosta-6,22-dien-3β,5α,8α-triol and brassicasterol.
Highlights
Fungi can be classified according to the type of host
Some compounds identified by gas chromatography with mass spectrometry (GC-MS) can be highlighted because they are common among the extracts of the analyzed fungi, such as long-chain fatty acids (9-hexadecenoic, hexadecanoic, oleic, octadecanoic, lauric, myristic, palmitic, docos-13-enoic, stearic, linoleic, and nonadecanoic acids) [17,18]
The results showed that the ethyl acetate extract of the mycelium inhibited the growth of all tested bacteria with promising activity to A. naeslundii (100 μg mL−1)
Summary
Fungi can be classified according to the type of host. Fungi that use host plants are called endophytic plants which do not cause plant diseases, while phytopathogens cause many types of diseases in theirs hosts [1], which is the case of some Cercospora species [2].Phytopathogenic fungi can produce toxic substances. Fungi that use host plants are called endophytic plants which do not cause plant diseases, while phytopathogens cause many types of diseases in theirs hosts [1], which is the case of some Cercospora species [2]. The toxins, are secondary metabolites produced by fungi, usually of low molecular mass (
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