Identification of stripe rust resistant genes in resistant synthetic hexaploid wheat accessions using linked markers

Abstract

Stripe rust, caused byPuccinia striiformisf. sp.tritici(Pst), is one of the most important diseases affecting wheat. In this study, seven gene-linked markers were used to identify the presence of stripe rust resistant genes in 51 accessions of synthetic hexaploid of wheat which were found to be resistant at seedling plant stage. Molecular marker-based gene identification showed the presence ofYr5,Yr10andYr15in three accessions,Yr36in three accessions,Yr48in seven accessions,YrR61in four accessions, andYrTP1in ten accessions of resistant hexaploid of wheat. These gene-linked markers were also used for the detection of genetic diversity. A total of 68 alleles were detected by these seven gene-linked markers. The mean number of allele was 11.3 alleles per locus. Genetic diversity values ranged from 0.34 to 0.93, with highest genetic diversity value of 0.93 detected for markerXwm477. The lowest genetic diversity value was observed for markerXbarc167. The polymorphic information content value ranged from 0.33 to 0.92 with an average of 0.54. The highest number of alleles (n= 24) were detected for markerXwmc477. The evidence in this study on the basis of genetic diversity and presence ofYrgenes in synthetic hexaploid wheat accessions will be useful in further breeding programmes.