Identification of SSR markers for hybrid purity testing in newly released rice hybrid KRH-4

Abstract

Microsatellite markers were used for fingerprinting of hybrid, assessing variation within parental lines and testing the hybrid purity of hybrid seed rice. In this study 25 simple sequence repeats (SSR) markers were employed for fingerprinting for newly release rice hybrid (KRH- 4) and their parental lines. Polymorphic primers viz., RM202, RM204, RM219, RM216, RM1385, RM21, RM336, RM209, RM7279 and RM206 could clearly distinguish KRH-4 from its parental lines. The polymorphic primer RM216 amplified an allele size of 160bp in CRMS32A (A) and 150bp in MSN36 (R). Similarly RM204 amplified an allele size of 110bp in CRMS-32A (A) and 120bp in MSN36 (R). RM7279 amplified an allele size of 190bp in CRMS32A (A) and 1800bp in MSN36 (R). Since the bands of RM204, RM216, and RM7279 of KRH-4 hybrid rice were complement type of their parents, clear and distinct that can be utilized for purity assessment of hybrid rice KRH-4.