Identification of SSR and RAPD markers linked to a resistance allele for angular leaf spot in the common bean (Phaseolus vulgaris) line ESAL 550

Gilvan Ferreira Da Silva,João Bosco Dos Santos,Magno Antonio Patto Ramalho

doi:10.1590/s1415-47572003000400009

Gilvan Ferreira Da Silva, João Bosco Dos Santos + Show 1 more

Open Access

https://doi.org/10.1590/s1415-47572003000400009

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Abstract

The objective of this study was to identify RAPD and SSR markers associated with a resistant allele for angular leaf spot (Phaeoisariopsis griseola) from the line 'ESAL 550', derived from the Andean 'Jalo EEP 558' cultivar, to assist selection of resistant genotypes. The resistant line 'ESAL 550' and the susceptible cultivar 'Carioca MG' were crossed to generate F1 and F2 populations. One hundred and twenty F2:3 families were evaluated. The DNA of the 12 most resistant families was bulked and the same was done with the DNA of the 10 most susceptible, generating two contrasting bulks. One RAPD and one SSR marker was found to be linked in coupling phase to the resistant allele. The SSR marker was amplified by the primer PV-atct001282C, and its distance from the resistant allele was 7.6 cM. This is the most useful marker for indirect selection of resistant plants in segregating populations. The RAPD marker was amplified by the primer OPP07857C linked in coupling phase to the resistant allele, and distant 24.4 cM. Therefore, this RAPD marker is not so useful in assisting selection because it is too far from the resistant allele.

Highlights

The consumption of common bean in Brazil has decreased more than 22% in the past 25 years, mainly due to irregular supplies and higher prices (Teixeira and Thung, 1994; Santos and Braga, 1998)
The phenotypic analysis of the 120 F2:3 families from the cross ‘ESAL 550’ x ‘Carioca MG’ presented a segregation of 90 resistant and 30 susceptible families, exactly three resistant to one susceptible (χ2 = 0, p = 100%), indicating a monogenic inheritance, with resistance being due to the dominant allele
Similar monogenic resistance was observed in the Andean cultivar AND 277 to the 63-23 race of P. griseola, and this resistant allele was tagged with a RAPD marker (Carvalho et al, 1998)

Summary

Introduction

The consumption of common bean in Brazil has decreased more than 22% in the past 25 years, mainly due to irregular supplies and higher prices (Teixeira and Thung, 1994; Santos and Braga, 1998). The bean crop faces several threats such as diseases, among which angular leaf spot, caused by the fungus Phaeisariopsis griseola, is one of the most important (Paula-Jr and Zambolim, 1998). The most efficient method of disease control is the use of resistant cultivars (Satorato and Rava, 1994; Paula-Jr and Zambolim, 1998). Some information on resistance sources and genetic control is already available, the procedures for artificial inoculation are not yet accurate resulting in low selection gain (Sartorato and Rava, 1994). An alternative is to select under natural incidence of the disease, which generally occurs only in the dry season, when the inoculum pressure is enough to assure efficient selection. The use of molecular markers for identifying vertical resistance alleles stands as an important contribution for bean breeding, mainly because they enable selection of genotypes with one or more resistance alleles at any time

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